A Comparison of Quantitative Composition and Bioactivity of Oils Derived from Seven North American Varieties of Hops (Humulus lupulus L.)

Seven commercial hop (Humulus lupulus L.) oils originating from a selection of North American varieties (Amarillo, Azacca, Cascade, Centennial, Chinook, Saaz, and Ahhhroma) six homemade hydrodistilled the same pellets (except were compared. terpenes regarded as oil markers (i.e., α-pinene, β-pinene, β-myrcene, β-ocimene, limonene, β-caryophyllene, α-humulene) methyl heptanoate identified quantified by GC–MS GC-FID. The antioxidant potential samples was evaluated using electron paramagnetic resonance (EPR) spectroscopy, while their components’ antibacterial (against Aliivibrio fischeri) enzyme (α-glucosidase lipase) inhibition activities screened high-performance thin-layer chromatography (HPTLC)-based assays. A distinct feature five Saaz relatively high contents β-myrcene (between 4.21 6.40 µg mg−1 oil). Centennial had perceptibly higher β-caryophyllene than rest, most them Chinook) contained amounts α-humulene. Differences between terpene profiles suggested that derived cones in process different hydrodistillation. showed low potential, comparable to popular beers white wines. highest observed Ahhhroma oil, it very no Cascade oils. developed streamlined workflow, including parallel HPTLC-directed bioassays HPTLC—TLC–MS Interface—SPME–GC–MS, enabled identification dimyrcenes, β-farnesene, 2-methylbutyl isobutyrate anti-obesity compounds weak components.